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Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

Article DOI: 10.3791/1599-v • 12:04 min • December 1st, 2009
December 1st, 2009 •
1,2, 1,2, 1,2, 1,2
1Department of Molecular and Microbiology, George Mason University, 2Krasnow Institute for Advanced Study, George Mason University

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In this video-article we present a method for the isolation and purification of Drosophila peripheral neurons using a fast magnetic bead assisted cell sorting strategy. RNA obtained from the isolated cells can be readily used for downstream applications including microarray analyses.

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Isolation Purification Drosophila Peripheral Neurons Magnetic Bead Sorting PNS Neurons Dendritic Arborization (da) Neurons Neuronal Development Dendrite Morphogenesis Functional And Molecular Levels Pure Population Low Occurrence Tough Chitinous Larval Cuticle Specific Cell Enrichment Antibody-coated Magnetic Beads Age-matched Third Instar Larvae Mouse CD8 Tagged GFP Fusion Protein (UAS-mCD8-GFP) Pickpocket (ppk)-GAL4 Driver Pan-da Neuron-specific GAL421-7 Driver Larval Cuticle Cell Types
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