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Preparation of Spinal Cord Slice for the Whole-Cell Patch-Clamp Recording in Motor Neurons (Video) | JoVE

Understanding Motor Neuron Responses to Spinal Cord Stimulation

Article DOI: 10.3791/200496-v • 01:15 min • September 8th, 2023
September 8th, 2023 •
1, 1, 2,3, 1,2
1National Engineering Research Center of Neuromodulation, School of Aerospace Engineering, Tsinghua University, 2IDG/McGovern Institute for Brain Research, Tsinghua University, 3School of Life Sciences, Tsinghua University

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Ex Vivo Préparation Coupe de moelle épinière Enregistrement patch-clamp de cellules entières Motoneurones Stimulation de la moelle épinière Fonction locomotrice Lésion de la moelle épinière Réponses électriques Comportements sensorimoteurs Caractéristiques des stimulus Réponses cellulaires Méthode de patch-clamp Caractéristiques électrophysiologiques Viabilité cellulaire Séparation de la moelle épinière Structure osseuse Potentiels d’action Protocole Résolution spatio-temporelle Mécanisme électrique
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