Journal
/
Biología
/
An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
JoVE Revista
Biología
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología
An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications

An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications

DOI:
10.3791/2154-v

•

10:59 min

•

October 23, 2010

•

, , ,
1Department of Biomedical Engineering,Cornell University, 2Current Address: Mechanical Engineering Department,Dogus University

Capítulos

  • 00:00Título
  • 00:21Subscription Locl
  • 00:46Introduction
  • 01:37Preparing the Hammocks
  • 02:53 Ex ovo Culturing of the Chicken Embryos
  • 05:26Microinjection into the Vasculature
  • 08:34Left Atrial Ligation
  • 09:53Representative Results
  • 10:30Conclusion

Summary

Traducción Automática

Traducción Automática

In this article, we present a simple methodology to enable long-term ex-ovo avian embryo culture. This technique is ideal for longitudinal experimentation requiring complete optical accessibility and/or sterile transportation in avian embryos.

Tags

Ex-ovo Chicken Embryo Culture SystemImagingMicrosurgery ApplicationsGenetic FactorsMicroenvironmental FactorsEmbryonic DevelopmentTherapeutic StrategiesImaging TechnologyBody Plan OrganizationEmbryonic MorphogenesisChicken EmbryosVertebrate Animal Model SystemCulture And Surgical ManipulationFilter Paper RingsOptical AccessCell PatterningFate StudiesCardiac MorphogenesisEggshell Window TechniqueHigh Resolution ImagingUltrasonographyShell-less Culture SystemEnvironmental Chamber

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Windowing Chicken Eggs for Developmental Studies

Bioelectric Analyses of an Osseointegrated Intelligent Implant Design System for Amputees

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Xenotransplantation of Human Stem Cells into the Chicken Embryo

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

Mouse Embryonic Development in a Serum-free Whole Embryo Culture System

An Ex vivo Culture System to Study Thyroid Development

Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture

Read Article