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Immunology and Infection

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JoVE Journal Immunology and Infection
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
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Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Article DOI: 10.3791/3400-v • 09:13 min • November 1st, 2011
November 1st, 2011 •
1, 1, 1, 1
1Department of Pathology, University of Texas Medical Branch

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Resumen
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The reverse genetics system for the Rift Valley fever virus MP-12 vaccine strain is a useful tool for creating additional MP-12 mutants with increased attenuation and immunogenicity. We describe the protocol to generate and characterize NSs mutant strains.

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Reverse Genetics NSs Gene Rift Valley Fever Virus MP-12 Strain Vaccine Safety Vaccine Efficacy Hemorrhagic Fever Neurological Disorders Blindness Abortion Fetal Malformation HHS/USDA Overlap Select Agent Risk Group 3 Pathogen Phlebovirus Bunyaviridae Family Virulent RVFV MP-12 Vaccine Strain Wild-type RVFV Strains ZH548 ZH501 M-segment L-segment S-segment RNA3 Functional Virulence Factor NSs RMP12-C13type In-frame Deletion Of NSs ORF Host Transcription Shut-off Interferon (IFN)-beta MRNA7,8 Double-stranded RNA-dependent Protein Kinase (PKR) Post-translational Level Degradation Of PKR
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