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Omprogrammering humane somatiske celler i induceret pluripotente stamceller (IPSC'er) under anvendelse af retroviral vektor med GFP

Article DOI: 10.3791/3804-v • 08:25 min • April 3rd, 2012
April 3rd, 2012 •
1, 1, 1
1Yale Stem Cell Center, Department of Genetics, Yale School of Medicine

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En fremgangsmåde til at generere humane inducerede pluripotente stamceller (IPSC'er) via retrovirus-medieret ektopisk ekspression af OCT4 er SOX2, KLF4 og MYC beskrevet. En praktisk måde at identificere menneskelige IPSC kolonier baseret på GFP-ekspression er også diskuteret.

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Stem Cell Biology menneskelige iPS celler omprogrammering Retrovirusvektorer og pluripotency
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