Pluripotency Markers Detection
Pluripotency Markers Detection - Related Videos
08:58
Induced Pluripotency
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2023
Induced pluripotent stem cells (iPSCs) are somatic cells that have been genetically reprogrammed to form undifferentiated stem cells. Like embryonic stem cells, iPSCs can be grown in culture conditions that promote differentiation into different cell types. Thus, iPSCs may provide a potentially unlimited source of any human cell type, which is a major breakthrough in the field of regenerative medicine. However, more research into the derivation and differentiation of iPSCs is still needed to...
08:45
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
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Cited by 22 •
2017
This study implemented whole genome sequencing for analysis of mutations in genes conferring antifungal drug resistance in Candida glabrata. C. glabrata isolates resistant to echinocandins, azoles and 5-flucytosine, were sequenced to illustrate the methodology. Susceptibility profiles of the isolates correlated with presence or absence of specific mutation patterns in genes.
05:44
The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
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Cited by 43 •
2016
Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.
10:49
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
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Cited by 38 •
2013
This procedure yields telencephalic neurons by going through checkpoints which are similar to those observed during human development. The cells are allowed to spontaneously differentiate, are exposed to factors which push them towards the neural lineage, are isolated, and are plated onto coverslips to allow for terminal differentiation and maturation.
13:23
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
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Cited by 13 •
2012
We present a protocol for efficient reprogramming of human somatic cells into human induced pluripotent stem cells (hiPSC) using retroviral vectors encoding Oct3/4, Sox2, Klf4 and c-myc (OSKM) and identification of correctly reprogrammed hiPSC by live staining with Tra-1-81 antibody.
FAQs
Using both marker classes strengthens interpretation because they report different cellular features. OCT4, SOX2, and NANOG are transcription factors, whereas SSEA-4 and TRA-1-60 are surface antigens. Measuring these proteins or related molecular signals together can help distinguish a marker pattern associated with maintained pluripotency from changes linked to differentiation or loss of the state.
The meaning of a result depends on the cellular state being examined. Detection of OCT4, SOX2, or NANOG, together with surface antigens such as SSEA-4 or TRA-1-60, supports assessment of an undifferentiated population. Changes in these markers can instead indicate differentiation or loss of pluripotency, making marker patterns useful for tracking state transitions.
Immunostaining assesses marker proteins in cells, flow cytometry measures marker signals across cell populations, and gene-expression assays examine transcripts. These approaches provide different readouts of the same biological question: whether pluripotency-associated features are present. The choice depends on whether the study focuses on cellular protein assessment, population-level measurement, or transcript-level analysis.
An assessment can be organized by selecting pluripotency-associated markers, choosing an assay that measures proteins or transcripts, and interpreting the resulting pattern against the biological state of the cells. Applying this framework across self-renewing, differentiating, and differentiated populations helps reveal whether marker expression is maintained, changing, or absent.
Marker detection provides molecular and cellular evidence about whether a culture retains the expected pluripotency-associated state. This is particularly relevant for embryonic stem cells and induced pluripotent stem cells, as well as organoids and differentiated populations. Such measurements help characterize starting or resulting cell populations before interpreting experiments or comparing biological outcomes.
These measurements connect cell-state characterization with broader experimental goals. Researchers can apply them in disease modeling, developmental studies, and regenerative research involving stem cells, organoids, or differentiated populations. The resulting marker profiles help relate observations to self-renewal, differentiation, or loss of pluripotency rather than treating all cell populations as equivalent.