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Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
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Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

Article DOI: 10.3791/3916-v • 05:32 min • May 28th, 2012
May 28th, 2012 •
1, 1
1Department of Microbiology, Immunology, & Cancer Biology, University of Virginia Health System

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We describe a modified hot aqueous-phenol extraction method for purifying lipopolysaccharide (LPS) from Gram-negative bacteria. Once extracted, the LPS can be subsequently analyzed by SDS-PAGE and visualized by direct staining or Western immunoblot.

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Purification Visualization Lipopolysaccharide Gram-negative Bacteria Hot Aqueous-phenol Extraction Lipid A Core Oligosaccharide O-antigen Units Virulence Pathogenesis Serotyping Clinical Isolates Antibodies Mutants Extraction Method Nucleic Acids Proteins Sodium Dodecy
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