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Intramikros for Imaging subcellulære strukturer i levende mus Uttrykke Fluorescent Proteiner

Article DOI: 10.3791/50558-v • 08:23 min • September 1st, 2013
September 1st, 2013 •
1,2, 1, 1, 1,3, 1
1Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch National Institute of Dental and Craniofacial Research, National Institutes of Health, 2Department of Biology, University of North Carolina at Chapel Hill, 3Department of Chemical & Biochemical Engineering and Department of Biomedical Engineering, Rutgers University

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Intramikroskopi er et kraftig verktøy som gjør det mulig å avbilde ulike biologiske prosesser i levende dyr. I denne artikkelen presenterer vi en detaljert fremgangsmåte for avbildning av dynamikken i subcellulære strukturer, slik som de sekretoriske granuler, i spyttkjertlene hos levende mus.

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Cellular Biology Mikroskopi konfokalmikroskopi fluorescens multiphoton eksocytose cellebiologi dyr biologi dyremodeller Intravital Mikros spyttkjertlene eksocytose In vivo avbilding
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