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Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies
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JoVE Revista Bioingeniería
Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

11,230 Views

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13:02 min

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July 15, 2013

DOI:

10.3791/50678-v

DOI:
10.3791/50678-v

•

13:02 min
July 15, 2013

•

2 Views
, , , ,
1Department of Electrical and Computer Engineering,University of California, Davis , 2Department of Chemical Engineering and Materials Science,University of California, Davis , 3Department of Biomedical Engineering,University of California, Davis

Summary

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We report on techniques to micropattern nanoporous gold thin films via stencil printing and photolithography, as well as methods to culture cells on the microfabricated patterns. In addition, we describe image analysis methods to characterize morphology of the material and the cultured cells using scanning electron and fluorescence microscopy techniques.

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Nanoporous GoldMicrofabricationNanomaterialsCell-material InteractionBiosensorsThin FilmsPhotolithographyStencil MasksSelf-assemblySurface AreaElectrical ConductivityCatalytic ActivityBioconjugationImmunostainingImage Processing

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Daggumati, P., Kurtulus, O., Chapman, C. A. R., Dimlioglu, D., Seker, E. Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies. J. Vis. Exp. (77), e50678, doi:10.3791/50678 (2013).

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