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Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Article DOI: 10.3791/51438-v • 11:46 min • May 26th, 2014
May 26th, 2014 •
*1, *1, 1, 1,2
1Language and Genetics Department, Max Planck Institute for Psycholinguistics, 2Donders Institute for Brain, Cognition and Behaviour
* These authors contributed equally

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Interactions between proteins are fundamental to all cellular processes. Using Bioluminescence Resonance Energy Transfer, the interaction between a pair of proteins can be monitored in live cells and in real time. Furthermore, the effects of potentially pathogenic mutations can be assessed.

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Protein-protein Interactions Live Cells Bioluminescence Resonance Energy Transfer BRET Assay Donor Luciferase Enzyme Acceptor Fluorescent Protein Renilla Reniformis Luciferase Yellow Fluorescent Protein (YFP) Distance-dependent Energy Transfer Cultured Mammalian Cells Fusion Proteins Weak Interactions Transient Interactions Biochemical Environment Yeast Two-hybrid Mass Spectrometry Proteomics Studies Mapping Interacting Regions
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