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Immunology and Infection

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High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability

Article DOI: 10.3791/52122-v • 09:05 min • November 21st, 2014
November 21st, 2014 •
1, 1, 1,2
1Department of Medicine - Division of Infectious Diseases, Vanderbilt University School of Medicine, 2Tennessee Valley Healthcare Systems, U. S. Dept. of Veterans Affairs

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Here we describe a method to visualize the oncogenic bacterial organelle known as the Cag Type IV Secretion System (Cag-T4SS). We find that the Cag-T4SS is differentially produced on the surface of H. pylori in response to varying conditions of iron availability.

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High Resolution Electron Microscopy Helicobacter Pylori Cag Type IV Secretion System Pili Iron Availability Gram-negative Bacterium Gastric Cancer Virulence Factor Cag-pathogenicity Island Type IV Secretion System CagA Translocating CagA And Peptidoglycan Host Epithelial Cells Cytokine Expression Proinflammatory Pathways Cytoskeletal Remodeling Oncogenic Cell-signaling Networks Macromolecular Machine Inner And Outer Membrane Extracellular Space
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