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JoVE Journal Neuroscience
Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane
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Journal (Neuroscience)
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Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane

Article DOI: 10.3791/52187-v • 07:32 min • March 16th, 2015
March 16th, 2015 •
1, 1, 1, 1
1Department of Otolaryngology-Head and Neck Surgery, University of California, San Francisco

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Resumen
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The described post-auricular surgical approach allows rapid and direct delivery into the mouse cochlear scala tympani while minimizing blood loss and animal mortality. This method can be used for cochlear therapy using molecular, pharmacologic and viral delivery to postnatal mice through the round window membrane.

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Surgical Method Virally Mediated Gene Delivery Mouse Inner Ear Round Window Membrane Gene Therapy Sensorineural Deafness Molecular Mechanisms Genetic Mechanisms Hearing Loss Vectors Cochlea Injury Post-auricular Surgical Approach Molecular Delivery Pharmacologic Delivery Viral Delivery Postnatal Day 10 And Older Mice Scala Tympani Blood Loss Animal Mortality Inner And Middle Ear Structures Neck Muscles Hearing Preservation Vesicular Glutamate Transporter 3 Knockout (VGLUT3 KO) Mice Congenital Deafness Adeno-associated Virus (AAV-1)
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