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Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
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Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

Article DOI: 10.3791/52345-v • 12:13 min • April 5th, 2015
April 5th, 2015 •
1, 1
1Department of Biochemistry and Microbiology, University of Victoria

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Here we present a cost-effective method for defining chemical-genetic interactions in budding yeast. The approach is built on fundamental techniques in yeast molecular biology and is well suited for the mechanistic interrogation of small to medium collections of chemicals and other media environments.

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Chemical Genetic Interactions Saccharomyces Cerevisiae Mode Of Action Bioactive Chemicals Gene Deletion Mutants Hypomorphic Essential Gene Mutants Chemical-gene Interactions Compound Mode Of Action Chemotherapeutic Agent 5-fluorouracil (5-FU) TRAMP RNA Exosome DNA Repair Enzymes Proliferation Microarray-based Bar-coding RNA Metabolism DNA Metabolism Validation Protocols High-throughput Screens
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