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Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy

Article DOI: 10.3791/52577-v • 06:51 min • February 1st, 2015
February 1st, 2015 •
1
1LOEWE Excellence Cluster for Integrative Fungal Research (IPF), Senckenberg Research Institute

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A convenient and powerful method for studying autophagy in Penicillium chrysogenum by using starvation pads (mixtures of agarose and tap water in a microscope slide containing a central cavity) is presented here.

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Autophagy Analysis Penicillium Chrysogenum Starvation Pads Fluorescence Microscopy Cellular Quality Control Systems Biologically Relevant Molecules Organelle Dynamics Protease Activity Surplus Organelle Degradation Autophagy Regulation Genetic Factors Environmental Factors Filamentous Fungus
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