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JoVE Journal Developmental Biology
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
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Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Article DOI: 10.3791/52732-v • 08:26 min • May 14th, 2015
May 14th, 2015 •
1, 1,2
1Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Center, 2Department of Surgery, Division of Neurosurgery, Toronto Western Hospital and University of Toronto

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The adult mammalian spinal cord contains neural stem/progenitor cells (NSPCs) that can be isolated and expanded in culture. This protocol describes the harvesting, isolation, culture, and passaging of NSPCs generated from the periventricular region of the adult spinal cord from the rat and from human organ transplant donors.

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Neural Stem Cells Progenitor Cells Isolation Periventricular Region Adult Rat Spinal Cord Human Spinal Cord Culture Growth Factor-enriched Medium Proliferation Differentiation Neurons Astrocytes Oligodendrocytes Enzymatic Dissociation Papain-EDTA Solution Mechanical Dissociation Density Gradient Single Cell Suspension Neurobasal Medium Epidermal Growth Factor (EGF) Basic Fibroblast Growth Factor (bFGF) Heparin Neurospheres Adherent Cultures Precursor Cells Lineage Restriction In Vitro Study Exogenous Factors Transplantation Regenerative Repair
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