Animal models are important tools for the evaluation of tissue-engineered grafts. This paper presents the protocol for preparing an electrospun biodegradable polymer graft for use in anterior cruciate ligament tissue engineering, as well as a surgical protocol for implantation in a rat model.
Leong, N. L., Kabir, N., Arshi, A., Nazemi, A., Wu, B. M., McAllister, D. R., Petrigliano, F. A. Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts. J. Vis. Exp. (97), e52797, doi:10.3791/52797 (2015).