Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Yuan-Jyue Chen; Sundipta D. Rao; Georg Seelig

doi:10.3791/53087

Journal

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Biología

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Plásmido derivado de ADN Strand Displacement Puertas de Redes de reacción de Ejecución Química

JoVE Revista
Biología

This content is Free Access.

JoVE Revista Biología

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plásmido derivado de ADN Strand Displacement Puertas de Redes de reacción de Ejecución Química

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14,103 Views

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07:50 min

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November 25, 2015

DOI:

10.3791/53087-v

DOI:

10.3791/53087-v

•

07:50 min

November 25, 2015

•

4 Views

Yuan-Jyue Chen¹, Sundipta D. Rao¹, Georg Seelig^1,2

¹Department of Electrical Engineering,University of Washington, ²Department of Computer Science & Engineering,University of Washington

Summary

This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.