Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Sarah J. Potter; Tony DeFalco

doi:10.3791/53262

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Usando Ex Vivo Culturas Upright Gotita de enteros fetales Órganos para estudiar los procesos de desarrollo durante Ratón Organogénesis

Using <em>Ex Vivo</em> Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

JoVE Revista
Biología del desarrollo

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JoVE Revista Biología del desarrollo

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Usando Ex Vivo Culturas Upright Gotita de enteros fetales Órganos para estudiar los procesos de desarrollo durante Ratón Organogénesis

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12,417 Views

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09:47 min

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October 21, 2015

DOI:

10.3791/53262-v

DOI:

10.3791/53262-v

•

09:47 min

October 21, 2015

•

1 Views

Sarah J. Potter¹, Tony DeFalco¹

¹Division of Reproductive Sciences,Cincinnati Children’s Hospital Medical Center

Summary

The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.