Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Improved Generation of Induced Cardiomyocytes…
JoVE Journal
Developmental Biology

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Developmental Biology
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function
Journal (Developmental Biology)
High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function
Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
Journal (Developmental Biology)
Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
 

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Article DOI: 10.3791/53426-v • 10:05 min • November 13th, 2015
November 13th, 2015 •
1, 1, 1, 1, 1, 1
1Department of Pathology and Laboratory Medicine, McAllister Heart Institute, University of North Carolina, Chapel Hill

Capítulos

Resumen
Automatic Translation

We describe here a protocol for the generation of iCMs using retrovirus-mediated delivery of Gata4, Tbx5 and Mef2c in a polycistronic construct. This protocol yields a relatively homogeneous population of reprogrammed cells with improved efficiency and quality and is valuable for future studies of iCM reprogramming.

Tags

Induced Cardiomyocytes Polycistronic Construct Gata4 Mef2c Tbx5 Cardiac Fibroblasts Regenerative Medicine Heart Disease Treatment Forced Expression Defined Factors Reprogramming Efficiency In Vitro G,M,T-transduced Fibroblasts Reprogramming Mechanisms Stoichiometry MGT Vector Reprogramming Process Cardiac Fibroblast Isolation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter