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CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Article DOI: 10.3791/53597-v • 11:38 min • February 29th, 2016
February 29th, 2016 •
*1,2, *1, 1, 1,3, 1, 1, 3, 2, 1, 1
1Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, 3Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia
* These authors contributed equally

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This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.

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CN-GELFrEE Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis Separations Technique Native Samples Non-covalent Macromolecular Interactions Protein Complexes Recovery And Resolution Complex Composition Stoichiometry High Recovery Low Sample Dilution Casting System Serological Pipette Hot Blade Support Stand Tubing Flow Control Valve Gradient Mixer Magnetic Stirrer Stir Bars Leaks De-ionized Water Tape Layers Glass Tube
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