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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering og flowcytometrianalyse gliom-infiltrerende perifere mononukleære blodceller
Invasion of Human Cells by a Bacterial Pathogen
Journal (Immunology and Infection)
Invasion of Human Cells by a Bacterial Pathogen
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions
Journal (Immunology and Infection)
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions
 
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Isolering og flowcytometrianalyse gliom-infiltrerende perifere mononukleære blodceller

Article DOI: 10.3791/53676-v • 12:52 min • November 28th, 2015
November 28th, 2015 •
1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan

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Præsenteres her er en enkel fremgangsmåde til isolering og flowcytometrisk analyse af gliom-infiltrerende perifert blod mononukleære celler, der giver tidsafhængige kvantitative data om antallet og aktiveringsstatus af immunceller ind i tidlige hjernetumor mikromiljø.

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Immunologi gliom perifere mononukleære celler (PBMC'er) galectin-1 (gal-1) GL26-Cit-gal1i GL26-Cit-NT Gr-1 naturlige dræberceller (NK-celler)
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