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Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy
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Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

Article DOI: 10.3791/53979-v • 08:03 min • May 23rd, 2016
May 23rd, 2016 •
1
1Institute of Genetic Medicine, International Centre for Life, Newcastle University

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Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.

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Cardiomyocyte DNA Synthesis Ploidy Quantification Cardiomyocyte Regeneration Turnover Nucleoside Labeling Flow Cytometry Neo Cardiomyocyte Nuclei Polyploidization Screening Tool Dynamics Of Cardiomyocyte Generation Quantification Of Neo Cardiomyocyte Generation Cellular Source Polyploidation EdU Injection Surgical Scissors Skin Incision Diaphragm Heart Dissection 15 ML Centrifuge Tubes PBS Petri Dishes Scalpel Forceps
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