Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Enrico Tatti; Boyd A. McKew; Corrine Whitby; Cindy J. Smith

doi:10.3791/54067

/

ADN-ARN simultánea extracción de los sedimentos costeros y cuantificación de 16S rRNA genes y las transcripciones de PCR en tiempo real

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

JoVE Revista
Medio ambiente

This content is Free Access.

JoVE Revista Medio ambiente

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

ADN-ARN simultánea extracción de los sedimentos costeros y cuantificación de 16S rRNA genes y las transcripciones de PCR en tiempo real

Please note that all translations are automatically generated. Click here for the English version.

17,514 Views

•

11:37 min

•

June 11, 2016

DOI:

10.3791/54067-v

DOI:

10.3791/54067-v

•

11:37 min

June 11, 2016

•

19 Views

Enrico Tatti¹, Boyd A. McKew², Corrine Whitby², Cindy J. Smith¹

¹Microbiology, School of Natural Sciences,National University of Ireland Galway, ²School of Biological Sciences,University of Essex

Transcripción

Summary

A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.