Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Mikko Juusola; An Dau; Lei Zheng; Diana Rien

doi:10.3791/54142

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Método electrofisiológico para registrar las respuestas de voltaje intracelular Drosophila Los fotorreceptores y interneuronas a la luz Estímulos En Vivo</em

Electrophysiological Method for Recording Intracellular Voltage Responses of <em>Drosophila</em> Photoreceptors and Interneurons to Light Stimuli <em>In Vivo</em>

JoVE Revista
Neurociencias

This content is Free Access.

JoVE Revista Neurociencias

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Método electrofisiológico para registrar las respuestas de voltaje intracelular Drosophila Los fotorreceptores y interneuronas a la luz Estímulos En Vivo</em

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18,651 Views

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11:42 min

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June 19, 2016

DOI:

10.3791/54142-v

DOI:

10.3791/54142-v

•

11:42 min

June 19, 2016

•

3 Views

Mikko Juusola^1,2, An Dau², Lei Zheng¹, Diana Rien^1,2

¹National Key Laboratory of Cognitive Neuroscience and Learning,Beijing Normal University, ²Department of Biomedical Science,The University of Sheffield

Summary

Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.