Repetitive Transcranial Magnetic Stimulation to the Unilateral Hemisphere of Rat Brain

Jaewon Beom; Jung Chan Lee; Jin Chul Paeng; Tai Ryoon Han; Moon Suk Bang; Byung-Mo Oh

doi:10.3791/54217

La estimulación magnética transcraneal repetitiva en el Hemisferio unilateral de cerebro de rata

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Repetitive Transcranial Magnetic Stimulation to the Unilateral Hemisphere of Rat Brain

La estimulación magnética transcraneal repetitiva en el Hemisferio unilateral de cerebro de rata

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05:47 min

October 22, 2016

DOI: 10.3791/54217-v

Jaewon Beom^1,2, Jung Chan Lee^2,3,4, Jin Chul Paeng⁵, Tai Ryoon Han⁶, Moon Suk Bang⁷, Byung-Mo Oh⁷

¹Department of Rehabilitation Medicine,Chungnam National University Hospital, Daejeon, ²Department of Biomedical Engineering,Seoul National University College of Medicine, ³Institute of Medical and Biological Engineering, Medical Research Center,Seoul National University, ⁴Department of Biomedical Engineering,Seoul National University Hospital, ⁵Department of Nuclear Medicine,Seoul National University Hospital, Seoul National University College of Medicine, ⁶Department of Rehabilitation Medicine,Gangwon Do Rehabilitation Hospital, ⁷Department of Rehabilitation Medicine,Seoul National University Hospital, Seoul National University College of Medicine

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Overview

This study investigates the application of unilateral repetitive transcranial magnetic stimulation (rTMS) in a rat model to explore molecular mechanisms. The technique allows for the examination of interhemispheric differences in response to rTMS.

Key Study Components

Area of Science

Neuroscience
Neurostimulation
Animal Models

Background

rTMS is a non-invasive brain stimulation technique.
It can help elucidate molecular responses in the brain.
Unilateral stimulation allows for targeted investigation of hemispheric differences.
Proper technique is crucial to avoid unintended stimulation of the entire brain.

Purpose of Study

To conduct unilateral rTMS in a small animal model.
To investigate molecular mechanisms affected by rTMS.
To provide insights into interhemispheric differences in brain activity.

Methods Used

Application of rTMS using a 25-mm figure-8 coil.
Placement of the coil 1 cm lateral to the vertex on the biauricular line.
Angulation of the coil at 45 degrees.
Monitoring body temperature using a rectal probe and homeothermic blanket.

Main Results

Successful application of unilateral rTMS in the rat model.
Demonstration of the procedure by an experienced technician.
Potential for exploring molecular events in response to rTMS.
Insights into the challenges faced by new users of the technique.

Conclusions

Unilateral rTMS is a valuable tool for neuroscience research.
It enables the study of interhemispheric differences in brain function.
Proper technique and monitoring are essential for successful outcomes.

Frequently Asked Questions

What is rTMS?

Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive method used to stimulate specific areas of the brain.

How is the rTMS coil positioned?

The coil is placed 1 cm lateral to the vertex on the biauricular line and angled at 45 degrees.

What precautions are taken during the procedure?

A rectal probe is used to monitor the rat's body temperature, maintaining it at 37 degrees Celsius.

Who demonstrates the rTMS procedure?

An experienced technician from the laboratory, Jin-Joo Lee, demonstrates the procedure.

What are the advantages of unilateral rTMS?

It allows for targeted stimulation and investigation of molecular mechanisms in one hemisphere of the brain.

What challenges do new users face with rTMS?

New users may struggle with the size of the coil and the timing of the motor threshold.

Aplicamos estimulación magnética transcraneal repetitiva (EMTr) al hemisferio unilateral del cerebro de rata, colocando una bobina en forma de 8 de 25 mm a 1 cm lateral al vértice en la línea biauricular y angulando la bobina 45°. Se utilizó un sistema interno de refrigeración por agua para la EMTr durante más de 20 minutos.

El objetivo general de este experimento es llevar a cabo una estimulación magnética transcraneal repetitiva unilateral, o EMTr, en un modelo animal pequeño. Este método puede ayudar a responder preguntas clave sobre los mecanismos moleculares de la EMTr, al permitir la estimulación hemisférica unilateral en el cerebro de rata. La principal ventaja de esta técnica es que brinda la oportunidad de explorar las diferencias interhemisféricas de los eventos moleculares, en respuesta a la EMTr unilateral.

Las personas nuevas en este método pueden tener dificultades porque la bobina de EMTr puede ser tan grande que estimula todo el cerebro, y porque la sincronización del umbral motor requiere mucho tiempo y es laboriosa. Un técnico experimentado de nuestro laboratorio, Jin-Joo Lee, hará una demostración del procedimiento. Colocar una sonda rectal en una rata macho adulta anestesiada para controlar la temperatura corporal, utilizando una manta homeotérmica para mantener la temperatura a 37 grados centígrados.

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