Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

Hananeh Fonoudi; Hassan Ansari; Saeed Abbasalizadeh; Gillian M Blue; Nasser Aghdami; David S Winlaw; Richard P Harvey; Alexis Bosman; Hossein Baharvand

doi:10.3791/54276

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La producción a gran escala de los cardiomiocitos de células madre pluripotentes utilizando un pequeño protocolo de diferenciación basada en la molécula altamente reproducible

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

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Biología del desarrollo

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JoVE Revista Biología del desarrollo

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

La producción a gran escala de los cardiomiocitos de células madre pluripotentes utilizando un pequeño protocolo de diferenciación basada en la molécula altamente reproducible

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10,515 Views

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12:21 min

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July 25, 2016

DOI:

10.3791/54276-v

DOI:

10.3791/54276-v

•

12:21 min

July 25, 2016

•

2 Views

Hananeh Fonoudi*^1,2,3,8, Hassan Ansari*^1,8, Saeed Abbasalizadeh¹, Gillian M Blue^6,7, Nasser Aghdami¹, David S Winlaw^6,7, Richard P Harvey^2,3,4, Alexis Bosman*^2,3, Hossein Baharvand*^1,5

¹Department of Stem Cells and Developmental Biology, Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology, ACECR, ²Developmental and Stem Cell Biology Division,Victor Chang Cardiac Research Institute, ³St. Vincent´s Clinical School, Faculty of Medicine,University of New South Wales, ⁴School of Biotechnology and Biomolecular Sciences,University of New South Wales, ⁵Department of Developmental Biology,University of Science and Culture, ⁶Heart Centre for Children,The Children´s Hospital at Westmead, ⁷Sydney Medical School,University of Sydney, ⁸Department of Developmental Biology,University of Science and Culture, Tehran, Iran

Transcripción

Summary

Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.