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The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation

Article DOI: 10.3791/54525-v • 09:10 min • October 13th, 2016
October 13th, 2016 •
1, 1
1Centre National de la Recherche Scientifique (UMR 8119), Centre de Neurophysique, Physiologie et Pathologie, Université Paris Descartes

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We show how to prepare oblique slices of the spinal cord in young mice. This preparation allows for the stimulation of the ventral roots.

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Oblique Spinal Cord Slices Ventral Root Stimulation Motoneural Identification Ventral Cells Axon Collaterals Dissection Steps P2 To P11 Mouse Low-sodium ACSF Perfusion Decapitation Skin Removal Cuts Through Shoulders And Rib Cage Isolation Of Vertebral Column And Ribs Silicon-filled Petri Dish Cold ACSF Carbogen Bubbling Laminectomy Of Dorsal Side Fine Scissors Cutting Bone From Rostral End
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