Factores artificiales de ingeniería para manipular específicamente empalme alternativo en células humanas
8,966 Views
•
10:06 min
•
April 26, 2017
DOI:
Summary
Automatically generated
Este informe describe un método bioingeniería para diseñar y construir nuevos factores de empalme artificiales (ASFS) que modulan específicamente el empalme de genes diana en células de mamífero. Este método se puede ampliar para diseñar diversos factores artificiales para manipular otros aspectos del metabolismo del RNA.
Videos relacionados
09:58
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
Videos relacionados
13688 Views
11:22
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
Videos relacionados
8838 Views
13:10
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
Videos relacionados
7320 Views
10:25
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Videos relacionados
4858 Views
12:04
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
Videos relacionados
3563 Views
08:53
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
Videos relacionados
2724 Views
08:05
Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
Videos relacionados
26078 Views
11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Videos relacionados
12920 Views
08:35
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Videos relacionados
5526 Views
01:32
RNA Splicing
Videos relacionados
56295 Views
Read Article
Cite this Article
Wei, H., Liu, Y., Wang, Y., Lu, Q., Yang, X., Li, J., Wang, Z. Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells. J. Vis. Exp. (122), e54967, doi:10.3791/54967 (2017).
Copy