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Biochemistry

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JoVE Journal Biochemistry
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
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Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Article DOI: 10.3791/54971-v • 08:53 min • January 11th, 2017
January 11th, 2017 •
1, 1
1Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology, Case Western Reserve University School of Medicine

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This paper describes a method for assessing the interactions and assemblies of integral membrane proteins in vitro with various partner factors in a lipid-proximal environment.

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Scaffold Liposomes Reconstitution Lipid-proximal Protein-protein Interactions In Vitro Mitochondrial Dynamics Accessory Lipids Native Confirmation Tethered Partners Defined Lipid Cofactors Mitochondrial Fission Machinery Protein Complexes Membrane Compartments Procedure Chloroform Solutions Lipid Film Residual Solvent Centrifugal Evaporator Buffer A Millimolar Concentration
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