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Journal / Immunology and Infection / Legionella pneumophila yttre membranvesiklar:…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Legionella pneumophila yttre membranvesiklar
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Legionella pneumophila yttre membranvesiklar: Isolering och analys av deras pro-inflammatoriska Potential på makrofager

Article DOI: 10.3791/55146-v • 08:35 min • February 22nd, 2017
February 22nd, 2017 •
1, 1, 1, 1, 1, 1,2,3
1Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg

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Här beskriver vi reningen av Legionella pneumophila (L. pneumophila) yttre membranvesiklar (OMV) från flytande kulturer. Dessa renade vesiklar används sedan för behandling av makrofager för att analysera deras pro-inflammatorisk potential.

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Infektion , Yttre membranvesiklar makrofager rening differentiell ultracentrifugering pro-inflammatoriskt svar bakteriell replikering
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