La visualización de la interacción proteína-proteína en las fracciones nucleares y citoplasmáticas de Co-inmunoprecipitación y<em> In Situ</em> Ligadura ensayo de proximidad
12,772 Views
•
10:05 min
•
January 16, 2017
DOI:
Summary
Automatically generated
Protein-protein interactions can occur in both the nucleus and the cytoplasm of a cell. To investigate these interactions, traditional co-immunoprecipitation and modern proximity ligation assay are applied. In this study, we compare these two methods to visualize the distribution of NF90-RBM3 interactions in the nucleus and the cytoplasm.
Videos relacionados
05:43
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Videos relacionados
8284 Views
13:10
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Videos relacionados
9963 Views
09:18
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
Videos relacionados
7430 Views
11:19
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
Videos relacionados
16018 Views
08:56
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
Videos relacionados
5829 Views
10:31
Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
Videos relacionados
13091 Views
11:15
Imaging Protein-protein Interactions in vivo
Videos relacionados
21311 Views
00:08
In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
Videos relacionados
7029 Views
08:27
Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays
Videos relacionados
1562 Views
09:39
Proximity Ligand Assay to Localize Proteins in DNA Damage Sites
Videos relacionados
420 Views
Read Article
Cite this Article
Zhu, X., Zelmer, A., Wellmann, S. Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay. J. Vis. Exp. (119), e55218, doi:10.3791/55218 (2017).
Copy