Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Joshua R. Porter; William G. Telford; Eric Batchelor

doi:10.3791/55219

Journal

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Genética

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Una sola célula de perfiles de expresión génica El uso de FACS y qPCR con las Normas Internas

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

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Genética

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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Una sola célula de perfiles de expresión génica El uso de FACS y qPCR con las Normas Internas

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16,351 Views

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10:50 min

•

February 25, 2017

DOI:

10.3791/55219-v

DOI:

10.3791/55219-v

•

10:50 min

February 25, 2017

•

6 Views

Joshua R. Porter¹, William G. Telford², Eric Batchelor¹

¹Laboratory of Pathology, Center for Cancer Research,National Cancer Institute, ²Experimental Transplantation and Immunology Branch, Center for Cancer Research,National Cancer Institute

Transcripción

Summary

We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.

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Una sola célula de perfiles de expresión génica El uso de FACS y qPCR con las Normas Internas

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Summary

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