Aislamiento de mitocondrias intactas de músculo esquelético por centrifugación diferencial para Medidas respirometría de alta resolución
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10:25 min
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March 08, 2017
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Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.
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Cite this Article
Djafarzadeh, S., Jakob, S. M. Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements. J. Vis. Exp. (121), e55251, doi:10.3791/55251 (2017).
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