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Zebrafisk

Article DOI: 10.3791/55507-v • 08:24 min • April 18th, 2017
April 18th, 2017 •
1, 2, 1,2
1Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus (UCAMC), 2Neuroscience Graduate Program, University of Colorado Anschutz Medical Campus (UCAMC)

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Dette manuskript beskriver metoder til elektrofysiologiske optagelser fra spinal neuroner i zebrafisk embryoner og larver. Præparatet fastholder neuroner in situ og ofte indebærer minimal dissektion. Disse fremgangsmåder tillader den elektrofysiologiske undersøgelse af en række spinale neuroner, fra den oprindelige erhvervelse elektrisk ophidselse gennem de tidlige larvestadier.

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Neuroscience zebrafisk spinale neuroner motoriske neuroner sensoriske neuroner Rohon-Beard celle CaP elektrofysiologi hel celle patch clamp
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