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DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum
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DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum

Article DOI: 10.3791/55740-v • 06:05 min • June 16th, 2017
June 16th, 2017 •
*1,2, *1, 1, 1, 1
1Department of Orthopedic Surgery, Harvard Medical School and Brigham and Women's Hospital, 2Division of Graduate Medical Sciences, Boston University
* These authors contributed equally

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Resumen
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Using a lipophilic 1,1'-Dioctadecy-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) staining technique, Ambystoma mexicanum can undergo vascular perfusion to allow for easy visualization of the vasculature.

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DiI Perfusion Vascular Visualization Ambystoma Mexicanum Regenerative Medicine Revascularization Fluorescent Microscope Axolotl Limb Regeneration Tail Heart Eyes Gills Organs Sedation Level Surgical Forceps Skin Incision Cartilage Plates Thoracic Cavity Connective Tissue Major Blood Vessels Pericardium Removal Heart And Aorta
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