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Bioengineering

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Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry

Article DOI: 10.3791/55785-v • 11:01 min • July 5th, 2017
July 5th, 2017 •
1, 1, 1, 1, 1, 2, 1,3,4, 1,2
1Department of Ophthalmology, Hamilton Eye Institute, University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences, University of Tennessee Health Science Center

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Millions of people suffer from retinal degenerative diseases that result in irreversible blindness. A common element of many of these diseases is the loss of retinal ganglion cells (RGCs). This detailed protocol describes the isolation of primary murine RGCs by positive and negative selection with flow cytometry.

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Primary Murine Retinal Ganglion Cells (RGCs) Isolation Flow Cytometry Retinal Ganglion Cell Field Visual Acuity Retinal Degenerative Disease Technique Fast Visible Reproducible Standardized Protocol Sumana Chintalapudi Krishna Murtheadol Immortalized Rat Retinal Ganglion Cell Line RGC Phenotype Vision Research Community Dr. Morales-Tirado Live Cells Highly Enriched Cell Population
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