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Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Article DOI: 10.3791/55946-v • 11:12 min • September 11th, 2017
September 11th, 2017 •
1, 1, 2, 1
1Department of Biology, Section for Functional Genomics and Center for Bacterial Stress Response and Persistence (BASP), University of Copenhagen, 2Department of Microbiology and Infection Control, Statens Serum Institut

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Here, the power of a transposon-mediated random insertion of a non-coding DNA element was used to resolve its optimal chromosomal position.

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Optimal Chromosomal Location DNA Element Escherichia Coli Transposon-mediated Approach Competition Experiments Whole Genome Sequencing DNA Replication Genetic Element Fittest Clone Growth Advantage E.coli DARS2 Region Electrocompetant Cells PNKBOR DARS2 Electroporation Chamber Bacterial Suspension LB Broth
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