Imaging Neural Activity in the Primary Somatosensory Cortex Using <em>Thy1</em>-GCaMP6s Transgenic Mice

Xiaojing Lin; Tingbao Zhao; Wenhui Xiong; Shaonan Wen; Xiaoming Jin; Xiao-Ming Xu

doi:10.3791/56297

Journal

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Neurociencias

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Imágenes de la actividad neuronal en la corteza somatosensorial primaria usando Thy1-ratones transgénicos GCaMP6s

Imaging Neural Activity in the Primary Somatosensory Cortex Using <em>Thy1</em>-GCaMP6s Transgenic Mice

JoVE Revista
Neurociencias

This content is Free Access.

JoVE Revista Neurociencias

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Imágenes de la actividad neuronal en la corteza somatosensorial primaria usando Thy1-ratones transgénicos GCaMP6s

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11,007 Views

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07:04 min

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January 07, 2019

DOI:

10.3791/56297-v

DOI:

10.3791/56297-v

•

07:04 min

January 07, 2019

•

10982 Views

Xiaojing Lin^1,2, Tingbao Zhao³, Wenhui Xiong¹, Shaonan Wen⁴, Xiaoming Jin¹, Xiao-Ming Xu¹

¹Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology,Indiana University School of Medicine ²Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA,General Hospital of Jinan Military Region ³Department of Orthopedics, Shandong Cancer Hospital,Shandong University ⁴Department of Neurobiology, Institute of Basic Medical Sciences,Academy Military Medical Sciences

Summary

Se describe un procedimiento experimental para medir la actividad neuronal a través de dos ventanas ópticas arriba bilateral corticies somatosensorial primaria (S1) en Thy1-GCaMP6s de ratones transgénicos usando fotones 2 (2P) microscopia en vivo.