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Immunology and Infection

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JoVE Journal Immunology and Infection
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
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Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Article DOI: 10.3791/56605-v • 07:50 min • December 8th, 2017
December 8th, 2017 •
1, 1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Recombinant prototype foamy virus integrase protein is often contaminated with a bacterial nuclease during purification. This method identifies nuclease contamination and removes it from the final preparation of the enzyme.

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Detection Removal Nuclease Contamination Purification Recombinant Prototype Foamy Virus Integrase Retrovirology Biochemistry Structure Dynamics Of Integration Technique Identification Removal DNA Nuclease Activity Protein Purification E.Coli Expressing Prototype Foamy Virus Integrase Sonication Ultracentrifuge Tube Centrifugation Supernatant FPLC Column Nickel-charged Resin Conductivity UV Absorbance
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