Aislamiento y medidas respiratoria de las mitocondrias de Arabidopsis thaliana
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January 05, 2018
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Como las mitocondrias son sólo un pequeño porcentaje de la célula de la planta, tienen que ser purificado para una gama de estudios. Las mitocondrias pueden ser aisladas de una variedad de órganos de la planta de homogeneización, seguido de centrifugación gradiente diferencial y densidad para obtener una fracción altamente purificada mitocondrial.
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Lyu, W., Selinski, J., Li, L., Day, D. A., Murcha, M. W., Whelan, J., Wang, Y. Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana. J. Vis. Exp. (131), e56627, doi:10.3791/56627 (2018).
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