Inmovilización de Caenorhabditis elegans para analizar el transporte intracelular en las neuronas
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October 18, 2017
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The overall goal of this time lapse recording is to observe the intracellular transport events in Caenorhabditis elegans neurons.This method can help answer key questions in the cell biology field such as axonal transport and interflagellar transport.The main advantage of this technique is that one can observe intracellular transport in C.Elegans without dissection.Start this experiment by growing transgenic C.elegans strain of interest at 20
Summary
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Elegans de Caenorhabditis (C. elegans) es un buen modelo para estudiar el transporte axonal e intracelular. Aquí describo un protocolo para el análisis del transporte axonal e intraflagelar en C. elegansy grabación en vivo .
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Cite this Article
Niwa, S. Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons. J. Vis. Exp. (128), e56690, doi:10.3791/56690 (2017).
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