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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Article DOI: 10.3791/56976-v • 09:01 min • March 26th, 2018
March 26th, 2018 •
1,2, 2,3, 4, 2, 2, 2, 2,5, 1,2, 1,2, 2
1Laboratoire d’hématologie, CHU Lille, 2Univ. Lille, Inserm, CHU Lille, UMR-S 1172 - JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, 3Service d’hématologie pédiatrique, hôpital Jeanne de Flandre, CHU Lille, 4Laboratoire d’hématologie CH Lyon Sud, 5Service des Maladies du Sang, hôpital Claude Huriez, CHU Lille

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Resumen
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We outline a protocol to detect simultaneous expression of leukemia stem cell markers on primary acute myeloid leukemia cells by flow cytometry. We show how to quantify three progenitor populations and a putative LSC population with increasing degree of maturation. We confirmed the presence of these populations in corresponding patient-derived-xenografts.

Tags

Flow Cytometry Leukemia Stem Cells Primary Acute Myeloid Leukemia Patient-derived-xenografts Diagnosis Follow-up Clinical Relevance Hematology/oncology Antibodies Bone Marrow Mononuclear Cells Red Blood Cells Granulocytes Ficol Density Gradient Buffy Coat Layer Sterile Tube Supernatants Serum Components
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