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Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis

Article DOI: 10.3791/57279-v • 10:22 min • April 3rd, 2018
April 3rd, 2018 •
*1,2, *3, 2, 1
1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 2Facultad de Medicina, Universidad Nacional Autónoma de México, 3Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro
* These authors contributed equally

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Here, we describe a protocol to obtain the lipid droplet index (LD index) to study the dynamics of triacylglycerols in cells cultured in high-throughput experiments. The LD index assay is an easy and reliable method that uses BODIPY 493/503. This assay does not need dispendious lipid extraction or microscopy analysis.

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Lipid Index Determination Liquid Fluorescence Recovery Fungal Pathogen Ustilago Maydis Nutritional Conditions Lipid Droplet Index Lipid Droplet Lipid Field Microplate Nitrogen Starvation Monica Montero Universidade Federale Do Rio De Janeiro Brazil Liquid Florescence Recovery Assay Saccharomyces Cerevisiae Collaboration Buffers And Solutions BO-DP Stock Solution Dimethyl Sulphoxide Minus 70 Degrees Celsius BO-DP Quenching Solution LFR-Assay Quenching Solut
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