Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

Chlo&#233; J Simard; Guillaume Pelletier; Luc H Boudreau; Etienne Hebert-Chatelain; Nicolas Pichaud

doi:10.3791/57376

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Medición de consumo de oxígeno mitocondrial en las fibras Permeabilized de Drosophila utilizando cantidades mínimas de tejido

Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

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Bioquímica
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JoVE Revista Bioquímica

Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

Medición de consumo de oxígeno mitocondrial en las fibras Permeabilized de Drosophila utilizando cantidades mínimas de tejido

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11,973 Views

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14:55 min

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April 07, 2018

DOI:

10.3791/57376-v

DOI:

10.3791/57376-v

•

14:55 min

April 07, 2018

•

11960 Views

¹Département de chimie et biochimie,Université de Moncton ²Département de biologie,Université de Moncton

Transcripción

The overall goal of this procedure is to evaluate mitochondrial respiration in the common fruit fly, Drosophila Melanogaster, using high resolution respirometry.This is achieved by first dissecting the Drosophila, to collect the thorax.The thoraces containing the flight muscles are then carefully permeabilized mechanically, with sharp forceps, and incubated with saponin, for further permeabilization.The thoraces are then transferred into the chambers of a respirometer, and mitochondrial oxygen consumption is measured with a combination of different substrates, uncouplers and inhibitors.Hi, I am Chlo

Summary

En este artículo se describe un método para medir el consumo de oxígeno mediante respirometría de alta resolución de tórax permeabilized de Drosophila. Esta técnica requiere una cantidad mínima de tejido en comparación con la técnica de aislamiento mitocondrial clásica y los resultados obtenidos son más fisiológicamente relevantes.