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Bioengineering

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JoVE Journal Bioengineering
Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting
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Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Article DOI: 10.3791/57474-v • 11:37 min • June 18th, 2018
June 18th, 2018 •
*1,2, *1,2, *1, 1,2, 1,2
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty, Rostock University
* These authors contributed equally

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Resumen
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This protocol illustrates a safe and efficient procedure to modify CD133+ hematopoietic stem cells. The presented non-viral, magnetic polyplex-based approach may provide a basis for the optimization of therapeutic stem cell effects as well as for monitoring the administered cell product via magnetic resonance imaging.

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MicroRNA Transfer Adult Bone Marrow-derived Hematopoietic Stem Cells Cell Engineering Magnetic Targeting Regenerative Medicine Non-viral Transfection Method Sauter's Method Modification Of Hematopoietic Stem Cells Mesenchymal Stem Cells Protocol Human Lymphocyte Medium Collagenase B DNase Flow Cytometric Analysis PBS EDTA Density Gradient Centrifugation Tube
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