Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / 基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体…
JoVE Journal
Genetics

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Genetics
基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Journal (Genetics)
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
Journal (Genetics)
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
 
Click here for the English version

基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体

Article DOI: 10.3791/57499-v • 07:18 min • May 15th, 2018
May 15th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

Capítulos

Resumen
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

本文介绍了一种用于裂变酵母靶基因随机诱变的详细方法。例如, 我们针对的是rpt4+, 它对19S 蛋白酶体的亚基进行编码, 并筛选出破坏染色质的突变。

Tags

遗传学 问题 135 随机突变 裂变酵母 染色质 易出错 PCR 着丝粒 基因靶向
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter