Modelado de Osteosarcoma usando las células de vástago de Pluripotent inducido derivados del paciente el síndrome de Li-Fraumeni

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Citado 14 veces

08:52 min

13 de junio de 2018

10.3791/57664-v

13 de junio de 2018

8.4K vistas

Ruoji Zhou¹^,² , An Xu¹ , Jian Tu¹^,³ , Mo Liu¹ , Julian A Gingold⁴ , Ruiying Zhao¹ , Dung-Fang Lee¹^,²^,⁵^,⁶

¹Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, ²Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, ³Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, ⁴Women's Health Institute, Cleveland Clinic Foundation, ⁵Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, ⁶Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

Aquí, presentamos un protocolo para la generación de pluripotentes inducidas (iPSCs) de células de fibroblastos derivados pacientes de síndrome de Li-Fraumeni (LFS), diferenciación de iPSCs a través de las células madre mesenquimales (MSCs) y osteoblastos, modelado en vivo tumorigenesis con osteoblastos derivados de paciente LFS.