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Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes

Article DOI: 10.3791/57768-v • 09:32 min • February 13th, 2019
February 13th, 2019 •
*1, *1, 1,2
1Department of Entomology, Purdue University, 2Purdue Institute for Inflammation, Immunology and Infectious Disease, Purdue University
* These authors contributed equally

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Protocols are described for assessing the toxicity of chemistries to immature and adult mosquitoes for development as new classes of larvicides, adulticides and endectocides. The protocols enable high-throughput testing of multiple chemistries at single-point dose and subsequent evaluation via dose response assay to determine toxicity on contact or via ingestion.

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Protocols Testing Toxicity Novel Insecticidal Chemistries Mosquitoes Insecticide Discovery Vector Control Fields Larvicide Adulticide Delivery Route Advantages Technique Unformulated Chemistries Multiple Species Vector Mosquitoes High Throughput Capacity Procedure Scientific Technician 24-well Tissue Culture Plate Analytical Balance Test Compound Sterile Double Distilled Water Stock Solution Serially Dilute Working Stock Solutions Concentrations Larvae Excess Water Test Chemistry Physical Damage Mortality
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