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Developmental Biology

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Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells
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Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells

Article DOI: 10.3791/57995-v • 05:43 min • July 27th, 2018
July 27th, 2018 •
1, 1, 1, 1
1MRC Centre for Regenerative Medicine, University of Edinburgh

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This protocol describes a semi-automated approach to produce hepatocyte-like cells from human pluripotent stem cells in a 96 well plate format. This process is rapid and cost-effective, allowing the production of quality assured batches of hepatocyte-like cells for basic and applied human research.

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Semi-automated Production Hepatocyte-like Cells Pluripotent Stem Cells 96 Well Format Rapid Technique Cost-effective Reduced Variation Mass Production Laminin 521 Solution Automatic Liquid Handling Dispenser 96 Well Plate Incubation Aspiration MTeSR1 Medium ROCK Inhibitor Y27632 Cell Seeding Cell Incubator
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