Amplificación de cerca integrales provirus VIH-1 para secuenciación de próxima generación
12,164 Views
•
10:18 min
•
October 16, 2018
DOI:
Summary
Automatically generated
La secuencia proviral individual completa (FLIPS) proporciona un método eficiente y de alto rendimiento para la amplificación y secuenciación de sola, cerca de larga duración (intactos y defectuosos) de provirus de HIV-1 y permite la determinación de su potencial capacidad de replicación. FLIPS supera limitaciones de anteriores ensayos diseñados para secuenciar la reserva latente de VIH-1.
Videos relacionados
11:10
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
Videos relacionados
12311 Views
10:10
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
Videos relacionados
8440 Views
13:58
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Videos relacionados
31581 Views
13:07
Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Videos relacionados
9302 Views
08:31
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Videos relacionados
10953 Views
N/A
Next-generation Viral RNA/DNA in situ Hybridization Applications in Human Immunodeficiency Virus/Simian Immunodeficiency Virus Research
Videos relacionados
2096 Views
05:45
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
Videos relacionados
8819 Views
15:49
Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
Videos relacionados
1400 Views
19:57
An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings
Videos relacionados
18541 Views
10:18
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Videos relacionados
12.2K Views
Read Article
Cite this Article
Hiener, B., Eden, J., Horsburgh, B. A., Palmer, S. Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing. J. Vis. Exp. (140), e58016, doi:10.3791/58016 (2018).
Copy